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M-GREEN YEAST & MOLD AGAR + CAF is a selective medium used for detection and enumeration of fungi in beverages.
Principal
Peptones, yeast extract and dextrose provide nutrients for growth. Magnesium sulphate and potassium phosphate are the buffering agents. Diastase is a mixture of enzymes which hydrolyze starch. Bromcresol green is a pH indicator. The low pH and the presence of chloramphenicol inhibit the bacterial contamination. Agar is the solidifying agent.
Technique
Filter the sample through a sterile membrane and transfer the membrane on the agar surface. Then, cover the plate with the lid, turn it upside down and incubate at 30-35°C for 48 hours and up to 5 days in an aerobic atmosphere with increased humidity.
Interpretation Of Results
After incubation, colonies appearing on the filter surface can be counted. Mold colonies appear green and filamentous, whereas yeast colonies are green and opaque.
Storage
10-25°C away from light, until
the expiry date on the label or until signs of deterioration or contamination
are evident
